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中文摘要：
鸭坦布苏病毒（DTMUV）感染引起产蛋鸭产蛋急剧下降。目前,没有预防该病的疫苗。为研究DTMUV DNA疫苗的免疫效果,本研究将DTMUV的E基因插入pCAGGS载体,构建了重组质粒pCAGGS-E。将pCAGGS-E转染293T细胞后,采用间接免疫荧光（IFA）和western blot检测E蛋白的表达情况。结果显示,两种方法均检测到了E蛋白的特异性表达。将pCAGGS-E用脂质体包裹后通过尾静脉注射免疫BALB/c小鼠,采用间接ELISA检测抗体的产生情况。结果表明,随着免疫次数的增加及免疫时间的延长,免疫小鼠的抗体滴度逐渐升高。本研究证明,编码E基因的重组质粒DNA免疫小鼠后能够诱导有效的免疫应答,为DTMUV DNA疫苗的研究奠定了基础。
英文摘要：
Duck Tembusu virus（DTMUV） infection leads to decrease dramatically in egg production of laying duck.However,no vaccine against DTMUV is available at present.To investigate the immunogenicity of DNA vaccine against DTMUV,the E gene of DTMUV was cloned into eukaryotic expression vector pCAGGS to construct pCAGGS-E and the expression of E protein was detected in pCAGGS-E transfected 293T cells by indirect immunofluorescence assay and western blot.Furthermore,the immunogenicity was evaluated in BALB/c mice with the liposome capsuled pCAGGS-E via intravenous injection.The specific antibodies were detected by indirect ELISA,which showed the antibody titers increased gradually after pCAGGS-E multiple inoculations.In conclusion,the pCAGGS-E was able to induced specific immune responses in mice.
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