将抗番鸭GPV单抗腹水采用透析法标记异硫氰酸荧光素(FITC),制备成抗GPV荧光抗体,研制检测GPV抗原的直接免疫荧光诊断方法。结果显示GPV荧光抗体仅与GPV阳性的组织切片或细胞呈现特异性荧光,与番鸭细小病毒(MPV)、番鸭呼肠孤病毒(MDRV)、鸭副粘病毒(DPMV)、鸭病毒性肝炎病毒(DHV)和正常番鸭组织切片不反应;与间接荧光方法的符合率为92.9%。表明GPV荧光抗体具有较好的特异性、敏感性和准确性,可用于临床快速诊断番鸭小鹅瘟病。
Monoclonal antibody against goose parvovirus derived from muscovy duck was used to mark with fluorescein iso- thiocyanate by dialysis to manufacture fluorescent antibody in orger to prepare direct fluorescence assay (DFA) for gosling plague virus. The results showed that GPV fluorescent antibody only reacted with GPV-- positive--tissue slice or cell to pres- ent specific fluorescent, but not react with muscovy duck parvovirus (MPV), muscovy duck reovirus(MDRV) .duck paramyxo- viruses (DPMV),duck hepatitis virus(DHV) and normal tissue. The coincidence of DFA and with indirect fluorescence assay (IFA) was 92.9% for detecting GPV. The results above indicated that GPV fluorescent antibody had good specificity, sensi- tivity and accuracy so as to diagnose muscovy duck gosling plague.