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中文摘要：
目的：应用高效液相色谱技术,建立复方利血平氨苯蝶啶片中利血平的含量测定方法。方法：采用Alltima C18色谱柱（250 mm×4.6 mm,5μm）,以含0.04%庚烷磺酸钠的50 mmol·min^-1磷酸二氢铵溶液（磷酸调至pH3.0）-乙腈（48∶52,v/v）为流动相,流速0.5 mL·min^-1,检测波长268 nm。结果：在本方法中,利血平的线性关系良好,r为0.9999;专属性强,利血平和药品中其它成分以及强制降解产物之间都有良好的分离度;精密度和稳定性良好,RSD均小于2%;加样回收率良好,百分回收率在99.88%-101.4%之间。结论：该方法准确、简单、快速,可作为复方利血平氨苯蝶啶片中利血平的含量测定方法。
英文摘要：
Objective：To establish an HPLC method for the determination of reserpine in compound hypotensive tablets.Methods：An Alltima C18 column（250 mm×4.6 mm,5 μm）was used for the separation,with 50 mmol·L^-1 ammonium dihydrogen phosphate containing 0.04% heptane sulfonic acid sodium salt（adjusted pH to 3.0 with H3PO4）-acetonitrile（48∶52,v/v）as the mobile phase at the flow rate of 0.5 mL·min^-1.The detection wavelength was 268nm.Results：The method showed good linearity with a correlation coefficient（r）of 0.9999;the specificity study showed satisfactory resolution between reserpine,other ingredients and forced degradation products;the precision and stability were satisfactory with all RSDs below 2%;and the spiked recovery ranged from 99.01% to 101.92%.Conclusion：This method is an accurate,fast and simple method for the determination of reserpine in compound hypotensive tablets.
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