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中文摘要：
目的：建立高效液相色谱-电化学（HPLC-ECD）法测定大鼠脑脊液中多巴胺的含量。方法：高效液相色谱采用WatersAtlantis T3色谱柱,以乙腈-缓冲液（75 mmol.L-1三水合醋酸钠,24 mmol.L-1无水柠檬酸溶液,1.2 mmol.L-1十二烷基硫酸钠溶液,0.5 mmol.L-1EDTA-2Na溶液）（20∶80）为流动相,流动相pH 5.4,流速0.32 mL.min-1。考察本方法的专属性、线性范围、精密度和回收率。进一步比较第一代（氟哌啶醇）及第二代（利培酮）抗精神病药对大鼠前额叶皮层（mPFC）和伏隔核（NAC）中多巴胺释放的影响。结果：多巴胺的保留时间为6.6 min,线性范围是0.2～20 nmol.L-1（r=0.9998）,日内与日间精密度均〈7.3%,回收率在101.4～106.2%之间。给予氟哌啶醇后,mPFC中多巴胺含量基本无变化,NAC中多巴胺显著升高;给予利培酮后,mPFC和NAC中多巴胺均显著升高。结论：该方法准确、灵敏、稳定,适用于脑微透析样品中多巴胺的定量分析。第二代抗精神病药物（利培酮）可能通过升高前额叶皮层多巴胺水平改善阴性症状及认知功能。
英文摘要：
Objective ： To develop an HPLC - ECD method for determination of dopamine in rat cerebral microdia- lysates. Methods： A Waters Atlantis T3 column was adopted in the HPLC system. The mobile phase was acetonitrile - buffer solution （75 mmol · L^- 1 sodium acetate trihydrate, 24 mmol · L^- 1 anhydrous citric acid, 1.2 mmol· L^- 1 dodecyl sulfate sodium salt and 0.5 mmol· L^- 11 EDTA - Na2 ） with pH 5.4（20： 80）. The flow rate was 0.32 mL ~ ~ -1 mm . Selectivity, linear range,precision and recovery were evaluated to investigate the reliability of this method, do- pamine was determined with microdialysates in order to study the effects of haloperidol and risperidone （ 1 mg · kg^- 1 ） s. c. on extraeellular dopamine levels in the mPFC and the NAC. Results： The retention time of dopamine was 6. 6 min ;Good linearity （r = 0. 9998）was obtained over the range of 0. 2 nmol · L^- 1-20 nmol · L^- 1 ;Intraday and interday precisions （RSDs）were tess than 7.3%. Haloperidol increased extracellular dopamine levels in the NAC, significantly but not in the mPFC. Risperidone significantly increased extracellular dopamine levels in both NAC and mPFC. Con- clusion： The HPLC - ECD method is rapid,sensitive and specific for quantitative determination of dopamine.
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