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混合滤白浓缩血小板质量研究？

ISSN号：1673-4130
期刊名称：《国际检验医学杂志》
时间：0
分类：R333.143[医药卫生—人体生理学;医药卫生—基础医学]
作者机构：[1]重庆市血液中心,重庆400015
相关基金：国家卫生公益性行业科研专项（200902008）.

作者：黎美君[1], 马姗姗[1], 李明海[1], 杨俊鸿[1], 代华友[1], 骆展鹏[1], 邹晓萍[1], 欧阳熊妍[1]

关键词：混合, 滤白浓缩, 血小板, pooled, leukocytes reduced, platelets

中文摘要：

目的：通过改进混合血小板制备工艺,并用血小板专用滤白滤器对混合血小板进行白细胞过滤后,评估两个厂家制备的混合滤白浓缩血小板的质量。方法从400 mL 新鲜全血中分离白膜,在（22±2）℃保存约16 h,6袋相同血型白膜汇集并分离出混合血小板,采用对照组和实验组两个厂家的血小板滤器过滤,检测过滤前后样品中血小板和白细胞计数、pH 值、低渗休克、血小板最大聚集率和 CD62p 阳性表达率。结果过滤前两组混合浓缩血小板质量均符合国标要求,血小板计数、pH 值、白细胞计数、CD62P 阳性率、最大聚集率差异均无统计学意义（P ＆gt;0.05）；但过滤后产品实验组和对照组的 pH 值、最大聚集率和血小板回收率分别为（6.53±0.60）vs（7.00±0.06）、（5.5±3.8）%vs（77.4±14.7）%、（86.8±4.3）%vs （90.6±2.7）%,差异有统计学意义（P〈0.05）；而两组残余白细胞计数、CD62p 阳性表达率和 PCR 分别为（3.00±4.00×10^6/袋 vs（2.00±3.00）＆#215;106/袋、（2.40±0.90）%vs（2.00±0.80）%、（7.30±5.90）%vs（5.60±3.70）%,差异无统计学意义（P ＆gt;0.05）。结论采用该制备方法经两组厂家血小板滤器过滤后,制备的混合滤白浓缩血小板均能满足现行国标要求,但实验组滤器对血小板 pH 和聚集功能有影响。

英文摘要：

Objective To evaluate the quality of pooled platelets leukocytes reduced after filtering out leukocytes using two manufacturers of leucocyte filters for pooled platelets and improving the preparation method.Methods Pooled platelets was prepared from 400 mL fresh whole blood by buffy coats（BC）method,after 1 6 h,（22±2）℃ holding period,pooled six bags of ABO-matched buffy coats.and then filtered with two manufacturers of leucocyte filter,divided into the control group and the experimental group. Before and after filtering,the numbers of platelet and leukocyte,pH,hypotonic shock response（HSR）,platelet aggregation and CD62p expression were detected.Results Before filtering leukocytes,the platelet quality of two groups achieved the requirements of Chinese standards.The numbers of platelet and leukocyte,pH,CD62p expression（%）and platelet aggregation showed no significant difference between two groups（P 〉0.05）.However,After filtering,the pH,platelet aggregation and the platelet recovery,the experimental group and the control group,were （6.53±0.60）vs（7.00±0.06）、（5.5±3.8）% vs （77.4±14.7）%,（86.8±4.3）%vs （90.6±2.7）%,showed significant differences （P〈0.05）;but the residual leukocytes,HSR and CD62p expression respectively were （3.00±4.00）×10 6/ bag vs （2.00±3.00）×10^ 6/ bag,（2.40±0.90）% vs （2.00±0.80）%,（7.30±5.90）% vs （5.60 ±3. 70）%,there were no significant differences（P 〉0.05）.Conclusion The quality of pooled platelets leukocytes is reduced,after filtering out leukocytes with two manufacturers of leucocyte filters and improving the preparation method,achieves the requirements of Chinese standards.However,the leukocyte filters of experimental group might have influence on platelet aggregation and pH.

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