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中文摘要：
目的：探讨适用于以微柱凝胶技术为基础的IgG抗A/B吸收试验。方法：选取30例IgG抗A及抗B起始效价从64～1024的血清样本,与A型或B型红细胞于37℃进行吸收试验,于5min、10min、30min、1h、2h、4h吸收时间后分离并测定吸收后血清的效价。结果：①IgG抗A和抗B均存在吸收效果伴随吸收时间变化的趋势,起始效价在128～1024基本上于2h后进入平台期。②IgG抗A和抗B起始效价为128与256,512与1024的时间变化趋势均差异有统计学意义（均P〈0.05）;IgG抗B起始效价为256与512、1024之间的时间变化趋势均差异有统计学意义（均P〈0.05）。③相同吸收时相的IgG抗A与抗B吸收效果之间差异无统计学意义（P〉0.05）。结论：在依赖微柱凝胶技术条件下,采取37℃2h吸收1次后再结合1次1～2h就能对起始效价小于1024的IgG抗A和抗B能使其充分吸收。
英文摘要：
Objective：To investigate the optimal protocol of IgG anti-A/B adsorption test based micro column gel technique.Method：30 random cases of serum samples with IgG anti-A or anti-B were collected and assimilated by the corresponding A or B cells,then the isolated serum post adsorption test contained the reaction time of 5 min,10 min,30 min,1 h,2 h,4 h,respectively.Result：①The alterative tendency of adsorption effects varied in the different adsorption time,and the platform stages of adsorption effects in the initial titration from 128 to 1 024 occurred after about 2 hours.②The tendency of adsorption effects along with time in IgG anti-A and anti-B with the initial titration of 128 were significant different from those initial titration of 256,512 and 1 024（P0.05）,the same was true of IgG anti-B with the initial titration between 256 and 512 or 1 024（P0.05）.③In the same adsorption time,the tendency of adsorption effects between IgG anti-A and IgG anti-B were no obvious difference（P0.05）.Conclusion：Combined an adsorption test with 2 hours in 37℃ and another adsorption test with 1 to 2 hour,the largest efficacy in adsorption test of IgG anti-A/B whose initial titration is beneath 1 024 can be obtained.
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