欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
为明确茉莉酸甲酯（methyl jasmonate,Me JA）诱导葡萄果实抗病反应的机制,以‘巨峰’葡萄悬浮细胞为试材,将其继代培养一次后分别在含10μmol·L^-1 Me JA的B5培养基和含100 nmol·L^-1隐地蛋白的B5培养基中（模拟病原菌接种）振荡培养15 d,每3 d测定1次细胞质量及抗病相关指标。结果显示：单一Me JA处理未激活葡萄悬浮细胞内的防卫反应;而单一隐地蛋白处理则可立即显著诱导葡萄悬浮细胞内源H2O2迸发,提升vv NPR1.1、PR1和PR2表达水平和植保素含量;经Me JA处理的葡萄悬浮细胞在添加隐地蛋白后,其细胞内出现较单一隐地蛋白处理更为显著的H2O2迸发、PR基因表达和植保素合成现象,说明经Me JA处理的悬浮细胞只在病原激发子胁迫时才表达出较强的系统抗性。因此,Me JA诱导的葡萄悬浮细胞抗病反应可归因于Priming（植物敏化过程或防御准备过程）机制。此外,经Me JA诱导的Priming反应对葡萄悬浮细胞生长无显著影响,暗示其未抑制葡萄细胞生长和胞内物质积累。
英文摘要：
In order to determine the mechanism underlying the Me JA（methyl jasmonate）-induced defense response in grapes,the grape cell suspensions from Vitis vinifera L. × V. labrusca L.‘Kyoho＇berries with being subcultured once were served as materials. The grape cells were shaking-cultured in the 100 m L B5 liquid medium,in which the Me JA were supplied to obtain the final concentration of 10 μmol · L^-1. Then the cell cultures in half of shake flasks were supplemented with 5 m L of 100 nmol · L^-1cryptogein solution for simulating pathogenic inoculation process and then incubated for 15 days. The cell growth,intracellular H2O2 burst,expression levels of defense-related genes such as Vv NPR1.1,PR1 and PR2 as well as contents of individual stilbene phytoalexins were measured at 3-day intervals. These results showed that treatment with 10 μmol · L^-1 Me JA alone could not activate the defense response in grape cells throughout the whole incubation. In contrast,the cryptogein treatment alone remarkably induced H2O2 burst and promoted the expressions of PR genes and contents of individual phytoalexins. Upon challenged with the cryptogein,the 10 μmol · L^-1 Me JA-treated cells displayed a series of more significant augmented defense responses including stronger H2O2 burst,more enhanced transcript levels of defense-related genes and phytoalexins biosynthesis compared with the reaction in cryptogein elicitation alone. Since the Me JA-treated cells only showed an enhanced capacity to augment systemic resistance with the present of pathogenic elicitor,it is thus clear that 10 μmol · L^-1 Me JA triggers a defense response that can be attributed to obvious Priming mechanism in grape cell suspensions,rather than direct inducible resistance. In addition,the Me JA-induced Priming defense did not exhibit the significant influence on the growth of grape cell suspensions during the whole incubation,indicating that Priming mechanism had no negative effect on cell growth and accumulation of intercellular compounds.
同期刊论文项目
同项目期刊论文
Response of direct or priming defense against Botrytis cinerea to methyl jasmonate treatment at diff
Effect of ethanol treatment on disease resistance against anthracnose rot in postharvest loquat frui
期刊信息
位置：