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维生素B1、B2和B3对石吊兰素与BSA结合作用的影响

ISSN号：1006-0456
期刊名称：《南昌大学学报：工科版》
时间：0
分类：O657.3[理学—分析化学;理学—化学]
作者机构：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047
相关基金：国家自然科学基金资助项目（31060210,21167013）;江西省自然科学基金资助项目（20114BAB204019）;国家重氟实验室基金资助项目（SKLF-ZZB-201305,SKLF-ZZA-201302,SKLF-KF-201203）.

关键词：石吊兰素, B族维生素, 荧光猝灭, 圆二色谱, lysionotin , B vitamins , fluorescence quenching, circular dichroism spectroscopy

中文摘要：

在模拟人体生理条件下，联合应用紫外-可见光谱法、荧光光谱法以及圆二色谱（CD）技术，研究了3种B族维生素（VB1、VB2、VB3）分别对石吊兰素与牛血清白蛋白（BSA）结合作用的影响。计算了3种维生素分别存在下石吊兰素-BSA体系的猝灭常数（KSV）、结合常数（K）、结合位点数（n）和结合距离（r）等参数，探讨了3种维生素对石吊兰素-BSA相互作用可能的影响方式。结果显示：VB1、VB2、VB3的加入使得石吊兰素-BSA体系的KSV和K增大，n基本不变，r稍有减小，表明VB1、VB2、VB3均能够增加石吊兰素-BSA体系的稳定性。VB。使得BSA-石吊兰素体系的主要作用力类型从以氢键和范德华力为主转变为以疏水作用力为主，而VB2、VB3的存在没有改变BSA-石吊兰素体系的主要作用力。CD分析结果表明：3种B族维生素的加入均能使BSA的α-螺旋含量有所增加。推断VB1、VB2、VB3与BSA的结合导致了BSA分子构象变化是维生素影响BSA-石吊兰素结合的主要方式。

英文摘要：

The influence of the three kinds of B vitamins,VBl,VB2 and VB3 on the interaction between BSA and lysionotin in physiological buffer （ pH = 7.4） was investigated by various spectroscopic methods including UV-vis absorption, fluorescence and circular dichroism （CD） spectroscopy. The binding parameters such as the Stern- Volmer dynamic quenching constant （ Ksv ）, binding constant （ K）, the number of binding sites （n） and binding distance（r） were calculated ,and the influence mode of the vitamins were explored. The results indicated that the values of Ksv and K were increased, r values were decreased,while the values of n remained unchanged in the presence of VBl ,VB2 and VB3 ,respectively,which means all of VB1 ,VB2 and VB3 could increase the stability of lysionotin- BSA system. The main acting force of lysionotin-BSA interaction was changed from hydrogen bonding and van der Waals forces to hydrophobic forces in the presence of VB1 ,whereas VB2 and VB3 did not change the type of acting force. The analysis of CD spectra showed the content of the α-helix of BSA was increased. The molecular conformational change of BSA induced by the vitamins-BSA binding was a main interferential quomodo of the B vitamins.

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