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中文摘要：
合成并表征了一种荧光素衍生物——硫代异氰酸苯酯荧光素（FHBS）；采用紫外-可见光谱和荧光光谱，在乙醇／4-羟乙基哌嗪乙磺酸（HEPES）（体积比1：1，pH=7．0）溶液中，研究了FHBS作为检测探针对Hg^2＋的识别性能．结果表明，该探针对Hg^2＋具有高选择性和灵敏性，其它阴、阳离子的存在不干扰其对汞离子的测定．另外，Hg^2＋的加入使探针溶液从无色变为亮黄色，荧光从无色变为亮绿色，因此该探针为Hg^2＋的比色／荧光双模式探针，并可用于细胞的荧光成像．
英文摘要：
Phenyl isothiocyanate fluorescein （FHBS）was synthesized and characterized. The recognition of FHBS for Hg^2＋ was studied using UV-Vis and fluorescence spectroscopy in ethanol-HEPES solution （volume ratio 1 ： 1 , pH = 7.0）. This probe exhibited high selectivity and sensitivity for Hg^2＋ than other ion metals and anions, and other metal ions and anions did not interfere with the detection of Hg^2＋. Addition of Hg^2＋ caused a visual color change from colorless to bright yellow and a fluorescence change from colorless to bright green, so FHBS can be developed as a eolorimetric and fluorometric dual-model probes for mercury （ H ） ion. This system was also applied in bioimaging.
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