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中文摘要：
背景：艾灸生成物的安全性研究是近年来灸法领域的关注热点，探讨艾烟成分对人体器官、组织、细胞的生物学效应是研究艾灸安全性的重要方向。研究表明艾烟溶液可引起肺泡Ⅱ型上皮细胞A549凋亡，但具体机制尚不清楚。目的：观察艾烟采集物对肺泡Ⅱ型上皮细胞A549生长抑制率、细胞内活性氧、超氧化物歧化酶的影响，探讨艾烟采集物对A549的氧化损伤机制。 方法：采用便携式空气采样器采集艾条燃烧产生的烟气颗粒物，加入DMEM培养基分别配制质量浓度0.5，0.4，0.3，0.2，0.1，0.05，0.025，0.01 g/L的艾烟溶液干预A549细胞。采用细胞计数试剂盒（CCK-8）检测细胞生长抑制率，2’，7’-二氯荧光黄双乙酸盐法观察细胞内活性氧信号强度，分光光度法测量并计算细胞超氧化物歧化酶活性。结果与结论：不同质量浓度艾烟溶液干预A549细胞4，6，8 h结果均显示，0.4，0.5 g/L艾烟溶液对A549细胞的生长抑制率高于0.01，0.025 g/L组（P＜0.05）。质量浓度0.05，0.1 g/L艾烟溶液可降低肺A549细胞内活性氧的含量，但差异无显著性意义（P＞0.05）；高质量浓度0.4 g/L艾烟溶液可增加肺A549细胞内活性氧水平（P＜0.01），降低超氧化物歧化酶活性（P＜0.01）。结果证实，艾烟对A549细胞具有生物学活性，对细胞的过氧化作用可能是高浓度艾烟溶液抑制A549细胞生长的重要机制。
英文摘要：
BACKGROUND：The safety study of moxa smoke has been the focus of attention in the moxibustion research in recent years. As an important direction, the effects of moxa smoke on the human organ, tissue and cel s have been discussed. Studies have shown that the solution of moxa smoke can induce apoptosis of human lung adenocarcinoma cel s （A549）, but the specific mechanism is stil unclear. OBJECTIVE：To observe the influences of moxa smoke on growth inhibition, intracel ular reactive oxygen species and superoxide dismutase of A549 cel s, and to explore the oxidative damage mechanism of moxa smoke on A549 cel s.METHODS：Moxa smoke was col ected by a portable air sampler, and dissolved in DMEM to different concentrations, as 0.5, 0.4, 0.3, 0.2, 0.1, 0.05, 0.025, 0.01 g/L, which was used to culture A549 cel s. Cel Counting Kit-8 method was employed to test cel growth inhibition rate, 2’,7’-Dichlorofluorescin diacetate method was employed to determinate reactive oxygen species intensity, and spectrophotometric method was employed to measure and calculate enzymatic activity of superoxide dismutase. RESULTS AND CONCLUSION：The results of different concentrations of smoke solution intervening A549 cel s in 4, 6 and 8 hours showed that the concentration of 0.4 g/L and 0.5 g/L on A549 cel s growth inhibition rate was higher than 0.01 g/L and 0.025 g/L （P〈0.05）. Low concentration of moxa smoke （0.05 g/L and 0.1 g/L） could decrease the intracel ular levels of reactive oxygen species, but there was no statistical y significant difference between them （P〉0.05）;high concentration of moxa smoke （0.4 g/L） could increase intracel ular levels of reactive oxygen species （P〈0.01）, and decrease the activity of superoxide dismutase （P〈0.01）. Moxa smoke is shown to have biological activity on A549 cel s, and the mechanism of high-concentration moxa smoke effect on cel growth inhibition may be attributed to peroxidation.
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