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Effect of Acupuncture-moxibustion on the Expression of IGF-1 and SOCS2 in Colonic Mucosa of Rats with Ulcerative Colitis

ISSN号：1672-3597
期刊名称：《针灸推拿医学：英文版》
时间：0
分类：R2-03[医药卫生—中医学]
作者机构：[1]Shanghai University of Traditional Chinese Medicine, Shanghai 201203, E R. China
相关基金：Fund Items： National Basic Research Program of China （973 Program, 2009CB522900）; Shanghai Rising-star Program （10QA1406100）; Shanghai Leading Academic Discipline Project （s30304）

作者：王晓梅[1], 关鑫[1], 季光[1], 施征[1], 吴焕淦[1]

关键词：结肠炎, 溃疡性, 针灸疗法, 胰岛素样生长因子I, 细胞因子信号转导抑制蛋白, Colitis, Ulcerative, Acupuncture-moxibustion Therapy, Insulin-Like Growth Factor I, Suppressor of Cytokine Signaling Proteins

中文摘要：

目的：观察针灸对溃疡性结肠炎（ulcerative colitis，UC）大鼠结肠黏膜胰岛素样生长因子1（insulin-like growth factor-1，IGF-1）和细胞因子信号转导抑制因子-2（suppressor of cytokine signaling，SOCS2）表达的影响，探讨针灸治疗UC的作用机理。方法：将大鼠随机分为正常组、模型组、隔药灸纽和电针组，每组8只，采用免疫学方法加局部刺激制备UC大鼠模型。隔药灸组和电针组分别进行隔药灸和电针治疗，每目1次，连续治疗14d。光镜观察大鼠结肠黏膜形态学变化，采用PAS—AB、HID—AB染色观察结肠黏膜粘蛋白，免疫组织化学技术检测大鼠结肠黏膜IGF-1和SOCS2表达。结果：光镜下可见UC大鼠结肠溃疡形成和炎症，结肠黏膜粘蛋白减少（P〈0．01），IGF-1表达升高（P〈0．01），SOCS2表达降低（P〈0．01）；隔药灸、电针治疗后，结肠黏膜病理损伤减轻，粘蛋白增加（P〈0．01），IGF-1表达降低（P〈0．01），SOCS2表达升高（P〈0．01）。结论：UC大鼠结肠黏膜粘蛋白分泌减少，IGF-1表达升高、SOCS2表达下降；隔药灸、电针能改善UC大鼠结肠黏膜损伤，调节结肠粘蛋白的分泌及IGF-1和SOCS2的表达。

英文摘要：

Objective： To observe the effect of acupuncture-moxibustion on the expression of insulin-like growth factor-I （IGF-I） and suppressor of cytokine signaling-2 （SOCS2） in colonic mucosa of rat models of ulcerative colitis （UC）, and explore the mechanism of acupuncture- moxibustion therapy in treating UC. Methods： The rats were randomized into a normal control （NC） group, a model control （MC） group, an herb-partitioned moxibustion （HPM） group and an electroacupuncture （EA） group, 8 in each group. The rat models of UC were established by immunological methods combined with local stimulation. The rats in the HPM and EA groups were given herb-partitioned moxibustion and electroacupuncture treatments respectively, once every day, lasting for 14 d. The morphological variations of rat＇s colonic mucosa were observed under light microscope; the colonic mucosal mucin was detected by PAS-AB and HID-AB staining methods; the expression of IGF-1 and SOCS2 was assayed by the immunohistochemical method. Results： In the rat models of UC, ulceration and inflammation of the colon were revealed by light microscope. The concentration of colonic mucosal mucin was reduced （P〈0.01）, while the expression of IGF-1 had an increase （P〈0.01）, and the expression of SOCS2 was reduced （P〈0.01）. After HPM or EA treatment, the pathological injuries of colonic mucosa had improved, the concentration of mucin increased （P〈0.01）, the expression of IGF-1 decreased （P〈0.01）, and the expression of SOCS2 increased （P〈0.01）. Conclusion： The secretion of mucosal mucin in rat UC decreased, the expression of IGF-1 was significantly higher, while the expression of SOCS2 was remarkably lower; both HPM and EA can help improve the damage of colonic mucosa in rat UC, and modulate the secretion of mucin, as well as regulate the expression of IGF-1 and SOCS2 in the colonic mucosa.

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