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Regulating Effects of Herb Cake-partitioned Moxibustion on the Expression of p53 and C-myc Protein in Rats with Ulcerative Colitis

ISSN号：1672-3597
期刊名称：《针灸推拿医学：英文版》
时间：0
分类：R2-03[医药卫生—中医学]
作者机构：[1]Shanghai Research Institute of Acupuncture and Meridian, Shanghai 200030, E R. China
相关基金：Fund Items： National Basic Research Program of China （973 Program, 2009CB522900）; Shanghai Leading Academic Discipline Project （S30304）

作者：赵天平[1], 王晓梅[1], 刘慧荣[1], 吴焕淦[1], 周爽[1]

关键词：结肠炎, 溃疡性, 间接灸, 基因, P53, 原癌基因蛋白质C—myc类, Colitis, Ulcerative, Indirect Moixbustion, Genes, p53, Proto-Oncogene Proteins C-myc

中文摘要：

目的：从p53基因、原癌基因蛋白质C—myc表达与溃疡性结肠炎发病关系角度，探讨隔药灸对p53、C—myc表达影响，进一步探讨隔药灸治疗UC作用机制。方法：采用免疫学方法加局部刺激制备溃疡性结肠炎大鼠模型。40只SD大鼠随机数字表法分为正常组、模型组、隔药灸组、温和灸组，每组10只。温和灸组，选取天枢、气海穴悬灸，每次每穴灸10min。隔药灸组选取天枢、气海穴隔药灸，每次每穴灸2壮。免疫组织化学法检测溃疡性结肠炎大鼠结肠组织中p53、C—myc表达。结果：模型组大鼠结肠组织中p53、C-myc的表达在阳性面积、强度、免疫组织化学（immunohistochemistry,mc）指数方面均显著上调，与正常组比较具有显著差异（P〈0．01）；隔药灸组、温和灸组治疗后结肠Np53、C—myc的表达下调，与模型组比较具有显著差异（P〈0．01）结论：p53与C．myc在UC发生、发展中具有重要作用，隔药灸对UC结肠组织p53、C-myc表达水平具有调节作用。

英文摘要：

Objective： To investigate the mechanism of herb cake-partitioned moxibustion treating ulcerative colitis （UC） from the relationship between expression of p53 and C-myc protein, and morbidity of UC. Methods： Rats model of UC was made with immune methods and local stimulation. Forty SD rats were divided into normal, model, herb cake-partitioned, and mild moxibustion group by a random number table, 10 rats in each group. Hanging moxibustion in the mild moxibustion group was applied to Tianshu （ST 25） and Qihai （CV 6） for 10 rain. Two moxa cones of herb cake-partitioned moxibustion were applied to the same acupoints respectively, in the herb cake-partitioned moxibustion group. Expression of p53 and C-myc protein was measured with immuno- histochemistical method in the colonic tissue of rats with UC. Results： Postive area, strength, and the immunohistochemistry index of the expression of p53 and C-myc protein were found more in the model rats than those in the normal rats （P〈0.01）, whereas less in the herb cake-partitioned moxibustion and mild moxibustion groups than those in the model group （P〈0.01）. Conclusion： p53 and C-myc play important roles in the morbidity and development of UC, and herb cake-partitioned moxibustion could regulate the expression of p53 and C-myc protein in the colonic tissue of UC rats.

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