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Moxibustion activates mast cell degranulation at the ST25 in rats with colitis

ISSN号：1007-9327
期刊名称：World Journal of Gastroenterology
时间：0
页码：3733-3738
分类：S852.4[农业科学—基础兽医学;农业科学—兽医学;农业科学—畜牧兽医] Q95-331[生物学—动物学]
作者机构：[1]Shanghai Institute of Acupuncture-Moxibustion and Meridians, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, China, [2]Research Centre of Interconnections between meridian and entrails, Anhui University of Traditional Chinese Medicine, Hefei 230038, Anhui Province, China
相关基金：Supported by National Basic Research Program of China （973 program） No. 2009CB522900; National Natural Science Foundation of China No. 30973785; Shanghai Leading Academic Discipline Project No. S30304; Shanghai Rising-Star Program No. 10QA1406100
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关键词： SD大鼠, 肥大细胞, 结肠炎, 颗粒, 色甘酸二钠, 激活, 生理盐水, 甲苯胺蓝, Disodium cromoglycate, Colitis, Mast cell, Moxibustion, ST25 acupoint

中文摘要：

瞄准：为了与 trinitro-benzene-sulfonic 在老鼠在 Tianshu (ST25 ) 在桅杆房间(MC ) 的形态学和功能上调查艾灸的效果，酸(TNBS ) 导致了大肠炎。方法：53 只男 Sprague-Dawley 老鼠的一个总数随机被划分成一个正常的组和试验性的组。在试验性的组，导致 TNBS 的大肠炎的一个老鼠模型被建立，并且老鼠然后随机被划分成一个模型组，艾灸组，加 disodium cromoglycate (M + DC ) 的艾灸加正常的组和艾灸盐(M + NS ) 组。在艾灸组的老鼠为 10 min 在双边的 ST25 收到了推迟的艾灸，一旦为 7 d 的一天。分别地，在 M + DC 和 M + NS 组的老鼠是有 disodium cromoglycate 和在双边的 ST25 盐的正常的 pretreated 并且然后并发地在艾灸组受到象老鼠的一样的处理。染色方法的 hematoxylin 曙红被用来观察冒号和 toluidine 的组织学改进蓝色的方法被用来在 ST25 acupoint 区域观察桅杆房间。结果：在在艾灸组的结肠的损害的改进被观察，在 ST25 acupoint 的 MC 的 degranulation 比率比在模型组在艾灸组是显著地更高的(流血的 45.91 trointestinal (UGIB ) 与那些相比变老 < 65 年。方法：医药记录和有在 2007-2009 期间承认的公开 UGIB 的 526 个连续病人的一个内视镜检查法资料库被考察。在在内视镜检查法以后的 30 d 以内的起始的演讲和临床的路线被获得。结果：65 年的 235 个病人的一个总数组成了老人口(吝啬的年龄吗？

英文摘要：

AIM： To investigate the effects of moxibustion on the morphology and function of mast cells （MC） at Tianshu （ST25） in rats with trinitro-benzene-sulfonic acid （TNBS）-induced colitis. METHODS： A total of 53 male Sprague-Dawley rats were randomly divided into a normal group and experimental group. In the experimental group, a rat model of TNBS-induced colitis was established, and the rats were then randomly divided into a model group, moxi-bustion group, moxibustion plus disodium cromoglycate （M ＋ DC） group and moxibustion plus normal saline （M＋ NS） group. Rats in the moxibustion group received suspended moxibustion at bilateral ST25 for 10 min, once a day for 7 d. Rats in the M ＋ DC and M ＋ NS groups were pretreated with disodium cromoglycate and normal saline at bilateral ST25, respectively, and were then concurrently subjected to the same treatment as rats in the moxibustion group. The hematoxy- lin-eosin staining method was used to observe histology of the colon and the toluidine blue-improved method was used to observe mast cells at ST25 acupoint areas. RESULTS： An improvement in colonic injury in the moxibustion group was observed and the degranulation ratio of MC at ST25 acupoint was markedly higher in the moxibustion group than in the model group （45.91 ± 11.41 vs 32.58 ± 8.28, P 0.05）. After inhibition of degranulation of MC at ST25 by disodium cromoglycate, no improvement in colon tissue injury was observed. CONCLUSION： Moxibustion exerted its effect on healing impaired colonic mucosa in rats with TNBS-induced colitis by increasing the degranulation ratio of local MC, but had little effect on the morphology of MC at ST25 acupoint.

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