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艾灸生成物干预大鼠尿液代谢组学研究

ISSN号：2095-4344
期刊名称：《中国组织工程研究》
时间：0
分类：R318[医药卫生—生物医学工程;医药卫生—基础医学]
作者机构：[1]上海中医药大学上海市针灸经络研究所,上海市200030, [2]上海中医药大学,上海市201203, [3]上海中医药大学附属岳阳中西医结合医院,上海市200437, [4]江西中医药大学,江西省南昌市330004
相关基金：国家自然科学基金项目（81102637）; 上海高校青年教师培养资助计划项目（szy11067）; 国家重点基础研究发展计划（973计划）（2009CB522900）; 上海市卫生局科研项目（20134239）

作者：周次利[1], 陆嫄[2], 吴璐一[2], 刘慧荣[1], 崔云华[1], 赵继梦[2], 李璟[3], 周志刚[2,4], 吴焕淦[1]

关键词：尿, 烟, 代谢, 组织构建, 组织工程, 艾灸生成物, 艾烟, 代谢组学, 尿液, 气相色谱-飞行时间质谱, 代谢途径, 大鼠, 国家自然科学基金

中文摘要：

背景：艾灸过程中产生的艾烟等艾灸生成物是灸法研究的热点之一。运用代谢组学理论与技术,可更为全面、系统地研究艾烟等艾灸生成物对机体的影响。目的：从尿液代谢组学角度观察不同浓度艾灸生成物对大鼠的影响。方法：40只SD大鼠随机等分为正常对照组、低、中、高剂量艾灸生成物组、高剂量艾灸生成物恢复组,低、中、高剂量艾灸生成物组和高剂量艾灸生成物恢复组大鼠在艾灸生成物与纯净气的浓度比分别为0.4∶2.0,0.8∶2.0,1.6∶2.0,1.6∶2.0中暴露4 h/d,每周5 d,持续60 d;高剂量艾灸生成物恢复组经60 d高剂量艾灸生成物刺激后,在空气中自然暴露21 d作为恢复期。正常对照组大鼠常规饲养60 d。结果与结论：气相色谱-飞行时间质谱联用仪分析各组大鼠尿液,共鉴定出108个代谢物,标准品库核实64个。各组大鼠尿液典型代谢物的变化与艾灸生成物浓度呈现一定的正相关关系,其中高剂量艾灸生成物组和正常对照组大鼠尿液代谢物差异最明显,葡萄糖醛酸、维生素C等22个差异代谢物主要参与维生素C代谢等15条糖、氨基酸相关代谢途径,提示艾灸生成物干预大鼠机体能量代谢增加,机体解毒、抗氧化作增强。

英文摘要：

BACKGROUND： Moxibustion products produced in moxibustion, such as moxa smoke, are one of hotspots in moxibustion research. Metabonomics can be used to more comprehensively and systematically study the effects of moxibustion products on the body. OBJECTIVE： To investigate the effect of different concentrations of moxibustion products on urine metabonomics of rats. METHODS： Forty Sprague-Dawley rats were randomly divided into normal control group, low-dose moxibustion products group, middle-dose moxibustion products group, high-dose moxibustion products group, high-dose moxibustion products recovery group. In the latter four groups, rats from each group were exposed to the mixtureof moxibustion products and pure gas at ratios of 0.4：2.0, 0.8：2.0, 1.6：2.0, 1.6：2.0, respectively, 4 hours daily, 5 days weekly, totally for 60 days. After 60-day high-dose moxibustion products stimulation, rats in the high-dose moxibustion products recovery group were raised in normal air for 21 days. Rats in the normal control group were raised in normal air for 60 days without any moxibustion products. Then we analyzed the changes of urine metabonomics in all group rats. RESULTS AND CONCLUSION： Totally 108 metabolites were identified in the urine of rats using gas chromatography/time-of-flight mass spectrometry, and 64 metabolites were verified by standard library. There were some positive correlations between changes of typical metabolites and moxibustion product concentrations. The metabolites in the urine were most different between the high-dose moxibustion products group and normal control group. Twenty-two differential metabolites, such as glucuronic acid and vitamin C were mainly involved in 15 sugar and amino acid metabolic pathways, such as ascorbate and aldarate metabolism. These findings indicated that energy metabolism, detoxification and anoxidation increased in rats stimulated by moxibustion products.

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