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针灸对克罗恩病大鼠结肠组织碱性成纤维细胞生长因子及Ⅰ型胶原的影响

ISSN号：1672-3597
期刊名称：《针灸推拿医学：英文版》
时间：0
分类：R2-03[医药卫生—中医学]
作者机构：[1]Shanghai University of Traditional Chinese Medicine, Shanghai 201203, E R. China, [2]Shanghai Research Institute of Acupuncture and Moxibustion, Shanghai 200030, E R. China, [3]Shanghai Research Institute of Qigong, Shanghai 200030, E R. China
相关基金：National Basic Research Program of China（973 Program,2009CB522900）;National Natural Science Foundation of China（30973785）;The Key Laboratory of Acupuncture-immune Effects of State Administration of Traditional Chinese Medicine

作者：赵琛[1], 丁菊英[2], 马金丹[1], 谭琳莹[2], 吴焕淦[2], 张英英[2], 张琳珊[3]

关键词：针灸疗法, 克罗恩病, 成纤维细胞生长因子, 碱性, Ⅰ型胶原蛋白, 纤维化, Acupuncture-moxibustion Therapy, Crohn Disease, Fibroblast Growth Factor,Basic, Collagen Type Ⅰ, Fibrosis

中文摘要：

目的：观察隔药灸、温和灸、电针对克罗恩病（Crohn＇s Disease,CD）大鼠结肠碱性成纤维细胞生长因子（Basic Fibroblast Growth Factor,bFGF）、Ⅰ型胶原,（Collagen Type Ⅰ,Coll）表达的调控作用,探讨针灸治疗克罗恩病肠壁纤维化的作用机制.方法：采用TNBS多次致炎方法制备大鼠克罗恩病模型.将大鼠随机分为正常组、模型组、温和灸组、电针组和隔药灸组.各针灸治疗组选取天枢、气海穴分别采用不同的针灸方法进行治疗.采用免疫组织化学法观察大鼠结肠组织Coll、bFGF蛋白的分布和表达.结果：模型组大鼠结肠组织Col Ⅰ、bFGF蛋白表达显著增强（与正常组相比,P〈0.01）;经过隔药灸、温和灸、电针治疗后,CD大鼠Col Ⅰ、bFGF蛋白表达明显减少（P〈0.01）.结肠组织bFGF与Col Ⅰ蛋白Spearman等级相关分析结果表明,两者具有明显相关性（P〈0.01）.结论：针灸可以下调CD大鼠结肠异常增高的Col Ⅰ、bFGF蛋白表达.bFGF、Col Ⅰ蛋白参与了大鼠CD肠纤维化过程,针灸可能通过降低结肠bFGF蛋白表达,从而调节Col Ⅰ蛋白的沉积而发挥对CD肠纤维化的治疗作用.

英文摘要：

Objective：To observe the impacts of herb-partitioned moxibustion,warm moxibustion and electroacupuncture on the basic fibroblast growth factor（bFGF）and collagen type Ⅰ（Col Ⅰ）in colons of rats with Crohn＇ disease（CD）,and discuss the mechanism of acupuncture therapy on the intestinal fibrosis in CD.Methods：The model rats were developed by TNBS as multiple proinflammatory method.The rats were randomly divided into 5 groups：a normal group,a model group,a warm moxibustion group,an electroacupuncture group and a herb-partitioned moxibustion group.The treatments were carried out at Tianshu（ST 25）（bilateral）and Qihai（CV 6）in different treatments.The immunohistochemistry was used to detect the expression position of Col Ⅰ and bFGF.Results：The expressions of Col Ⅰ and bFGF in colons of rots in the model group significantly increased（compared with the normal group,P〈0.01）.After the herb-partitioned moxibustion,warm moxibustion and electroacupuncture,the expressions of Col Ⅰ and bFGF reduced markedly in the rats with CD（P〈0.01）.The expression of bFGF and Col Ⅰ in the colons had an obvious correlation in the Spearman rank correlation analysis.Conclusion：Acupuncture treatment reduced the abnormally high levels of expressions for Col Ⅰ and bFGF in colons.Col Ⅰ and bFGF participated in the fibrosis.Acupuncture treatment may reduce the bFGF expression in colons to regulate the excessive deposition,treating the intestinal fibrosis in CD.

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