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不同灸温对急性佐剂性关节炎大鼠血清IL-1β、IL-2及TNF-α含量的影响

ISSN号：1005-0957
期刊名称：《上海针灸杂志》
时间：0
分类：R2-03[医药卫生—中医学]
作者机构：[1]南京中医药大学,南京210029
相关基金：国家重点基础研究发展计划项目（2009CB522900）：江苏省普通高校研究生科研创新计划项目（CXLX13-58）

关键词：针灸疗法, 灸法, 灸温, 佐剂性关节炎, 细胞因子, 大鼠, IL-1Β, IL-2, TNF-Α, Acupuncture-moxibustion therapy, Moxibustion method, Moxibustion temperature, Adjuvant arthritis, Cytokine, Rat

中文摘要：

目的：观察不同灸温下艾灸对于急性佐剂性关节炎大鼠血清IL-1β、IL-2及TNF-α含量的影响,为艾灸的局部抗炎免疫作用机制提供依据。方法采用弗氏完全佐剂（FCA）造模法制备大鼠佐剂性关节炎模型（AA）。将32只SD大鼠随机分出8只作为正常组,其余24只均造模处理,造模成功后随机分为模型组、治疗1组和治疗2组,每组8只。治疗1组和治疗2组均采用灸法治疗,治疗1组将施灸穴位局部温度控制在（38±1）℃,治疗2组将施灸穴位局部温度控制在（45±1）℃。采用酶联免疫法（ELISA）检测大鼠血清 IL-1β、IL-2及TNF-α含量。结果模型组、治疗1组和治疗2组血清IL-1β、IL-2及TNF-α含量与正常组比较,差异均具有统计学意义（P＜0.01）。治疗1组血清 IL-2含量与模型组比较,差异具有统计学意义（P＜0.01）。治疗2组血清IL-1β、IL-2及TNF-α含量与模型组和治疗1组比较,差异均具有统计学意义（P＜0.01）。结论艾灸具有降低血清中IL-1β、TNF-α含量,提高IL-2含量作用,且45℃灸温能够提高艾灸的作用。艾灸抗炎机制可能是通过降低血清中IL-1β、TNF-α含量,提高IL-2含量来缓解机体炎症反应,且艾灸作用发挥需要适宜的灸温。

英文摘要：

Objective To investigate the effect of different temperature moxibustion on serum IL-1β, IL-2 and TNF-αcontents in acute adjuvant arthritis rats and provide a basis for the mechanism of local anti-inflammatory and immune action of moxibustion. Methods A rat model of adjuvant arthritis （AA） was made by Freund’s complete adjuvant （FCA） modeling method. Of 32 SD rats, 8 were randomly selected as a normal group and the other 24 for model making. After successful model making, the rats were randomly allocated to model, treatment 1 and treatment 2 groups, 8 rats each. Treatment 1 and 2 groups received moxibustion. Local temperature at moxibustion point was controlled at （38±1）℃in treatment 1 group and at （45±1）℃in treatment 2 group. Rat serum IL-1β, IL-2 and TNF-αcontents were measured by enzyme-linked immunosorbent assay （ELISA）. Results There were statistically significant differences in serum IL-1β, IL-2 and TNF-αcontents between the model, treatment 1 or treatment 2 group and the normal group （P〈0.01）. There was a statistically significant difference in serum IL-2 content between treatment 1 and the model groups （P〈0.01）. There were statistically significant differences in serum IL-1β, IL-2 and TNF-αcontents between treatment 2 group and the model or treatment 1 group （P〈0.01）. Conclusions Moxibustion has a reducing effect on serum IL-1βand TNF-αcontents and a raising effect on IL-2 content. 45℃moxibustion temperature can improve the effect of moxi bustion. The anti-inflammatory action of moxibustion may be through the mechanism of reducing serum IL-1βand TNF-αcontents and raising IL-2 content, which relieve body inflammatory reaction. The action of moxibustion needs proper moxibustion temperature.

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