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中文摘要：
目的：研究对苯二酚在碱性条件下产生半醌自由基及其对耐甲氧西林的金黄色葡萄球菌（MRSA）、甲氧西林敏感葡萄球菌（MSSA）、BL21大肠杆菌3种菌的抑菌活性。方法：应用电子自旋共振（ESR）检测半醌昆自由基；应用高效液相色谱法（HPLC）检测对苯二酚在碱性条件下的反应产物；应用打孔法，研究对苯二酚在碱性条件下对MRSA、MSSA、BL21大肠杆菌3种菌的抑菌作用，并采用了96孔板法研究其对这3种菌的最小抑菌浓度（MIC）；同时通过测量这3细菌的生长曲线来比较对苯二酚在pH=7．5与pH=6条件下的抑菌活性。结果：半醌自由基抑菌作用较对苯二酚和对苯二酚在碱性条件下产生的最终产物都强。对苯二酚碱溶液对MRSA、MSSA、BL21大肠杆菌的MIC值分别为0．081、0．081、0．649mg／mL，而对苯二酚水溶液对MRSA、MSSA、BL21的MIC值分别为0．325、0．325、1．298mg／mL。结论：对苯二酚在碱性条件下的抑菌作用远远强于其在中性或者偏酸性条件下。
英文摘要：
Objective： To study hydroquinone producing semiquinone radicals in alkaline conditions and il：s antimicrobiat effects on methicillin-resistant staphylococcus aureus （MRSA）, methicillin sensitive staphylococcus aureus （MSSA） and escherichia coli （BL） 21. Methods： Electron spin resonance method was used in testing the semiquinone radical. High performance liquid chromatography （HPLC） method was used in testing the reaction products of semiquinone radicals in alkaline cinditions. Punching method was used in each species of MRSA, MSSA and BL21 to determine the antimicrobial effects of hydroquinone in alkaline conditions, and the method of 96-well plates was used to determine its minimal inhibitoryconcentration （MIC）. At the same time, the three bacteria growth curve were measured and compared to antimicrobial activity about hy droquinone in pH=7.5 and pH=6 condition. Results： The antimicrobial effects of the semiquinone radicals were stronger than those of hydroquinone and its final products in alkaline conditions. The values of MIC that hydroquinone alkaline solution inhibited MRSA, MSSA and BL21 were respectively 0.081, 0.081 and 0.649mg/mL, while the hydroquinone solution 0.325, 0.325 and 1.298mg/mL. Conclusion： Hydroquinone antimicrobial activity in alkaline conditions was stronger than that in neutral or acid conditions.
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