中文摘要：

瞄准：在 interleukin-12 (IL-12 ) 和肿瘤坏死因素的肠的植物群和版本上调查艾灸的效果 --(TNF-) 从在有 ulcerative 大肠炎(UC ) 的老鼠的冒号。方法：UC 的一个老鼠模型被肠的本地刺激从从人的 UC 病人收获的结肠的内容与上层清液建立。40 只男 Sprague-Dawley 老鼠的一个总数随机被划分成下列组：正常(假冒) ，模型(UC ) ，植物分区艾灸(对待 HPM ) ，并且积极控制 sulfasalazine (对待 SA ) 。与 HPM 对待的老鼠在针灸点 ST25 和 RN6 收到了 HPM 为 15 min 的一天，为 8 d 的一个总数。在 SA 组的老鼠一天为 8 d 与 SA 被酒两次。结肠的组织病理学说被 hematoxylin 曙红观察。肠的植物群的层次包括 Bifidobacterium，乳杆菌， Escherichia coli (E。coli ) ，并且 Bacteroides fragilis (B。fragilis ) ，被即时量的聚合酶链反应测试检测细菌的 16S rRNA/DNA 以便决定每特定的种的 DNA 拷贝数字。Immunohistochemical 试金被用来在老鼠冒号观察 TNF-12 和 IL-12 的表示。结果：HPM 治疗在 UC 老鼠的结肠的纸巾禁止了 immunopathology；一般词法分数和 immunopathological 分数显著地与模型组相比在 HPM 和 SA 组被减少[ 3.5 ( 2.0-4.0 )， 3.0 ( 1.5-3.5 )对 6.0 ( 5.5-7.0 )， P < 0.05 为将军词法 20 ，和 3.00 ( 2.00-3.50 )， 3.00 ( 2.50-3.50 )对 5.00 ( 4.50-5.50 )， P < 0.01 为 immunopathological 得分]。是由 DNA 拷贝数字测量了，我们发现 Bifidobacterium 和乳杆菌，与一个健康冒号被联系，比在模型组在 HPM 和 SA 组是显著地更高的(1.395 ? 跱?

英文摘要：

AIM： To investigate the effect of moxibustion on intestinal flora and release of interleukin-12 （IL-12） and tumor necrosis factor-α （TNF-α） from the colon in rat with ulcerative colitis （UC）. METHODS： A rat model of UC was established by local stimulation of the intestine with supernatant from colonic contents harvested from human UC patients. A total of 40 male Sprague-Dawley rats were randomly divided into the following groups： normal （sham）, model （UC）, herb-partition moxibustion （HPM-treated）, and positive control sulfasalazine （SA-treated）. Rats treated with HPM received HPM at acupuncture points ST25 and RN6, once a day for 15 min, for a total of 8 d. Rats in the SA group were perfused with SA twice a day for 8 d. The colonic histopathology was observed by hematoxylin-eosin. The levels of intestinal flora, including Bifidobacterium, Lactobacillus, Escherichia coli （E. coli）, and Bacteroides fragilis （B. fragilis）, were tested by real-time quantitative polymerase chain reaction to detect bacterial 16S rRNA/DNA in order to determine DNA copy numbers of each specific species. Immunohistochemical assays were used to observe the expression of TNF-α and IL-12 in the rat colons. RESULTS： HPM treatment inhibited immunopathology in colonic tissues of UC rats; the general morphological score and the immunopathological score were significantly decreased in the HPM and SA groups compared with the model group [3.5 （2.0-4.0）, 3.0 （1.5-3.5） vs 6.0 （5.5-7.0）, P 〈 0.05 for the general morphological score, and 3.00 （2.00-3.50）, 3.00 （2.50-3.50） vs 5.00 （4.50-5.50）, P 〈 0.01 for the immunopathological score]. As measured by DNA copy number, we found that Bifidobacterium and Lactobacillus, which are associated with a healthy colon, were significantly higher in the HPM and SA groups than in the model group （1.395 ± 1.339, 1.461 ± 1.152 vs 0.045 ± 0.036, P 〈 0.01 for Bifidobacterium, and 0.395 ± 0.325, 0.851 ± 0.651 vs 0.0015 ± 0.0014, P 〈 0.0