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14-3-3sigma增强胰腺癌细胞株PANC-1的增殖能力

ISSN号：1007-8118
期刊名称：《中华肝胆外科杂志》
时间：0
分类：R73-36[医药卫生—肿瘤;医药卫生—临床医学]
作者机构：[1]华中科技大学同济医学院附属同济医院胆胰外科中心,武汉430030, [2]贵阳医学院附属医院肝胆外科
相关基金：国家自然科学基金面上项目（81071775,81172064,81160311）;国家自然科学基金青年基金（81001068,81101621）

关键词：胆管肿瘤, 微RNAS, 肿瘤浸润, 凋亡, Bile duct neoplasms , MicroRNAs , Neoplasm invasiveness, Apoptosis

中文摘要：

目的检测人胆管癌中差异性微RNA（miR）的表达,探讨其对胆管癌凋亡、侵袭转移特性的影响.方法利用基因芯片对胆管癌及癌旁组织进行差异miR筛选,利用实时PCR在11例胆管癌和癌旁组织标本中对差异基因miR-200a/b/c/141进行验证.利用Transwell实验对miR-200bmimics干预后的人胆管癌细胞系QBC939进行侵袭能力检测.结果 miR芯片显示,与癌旁组织相比,胆管癌组织中筛选到21条差异表达miR,其中15条高表达,6条低表达.RT-PCR验证提示,miR-200b在胆管癌组织中表达显著低于正常胆管组织及癌旁组织,胆管癌中miR-200b水平较癌旁组织中低（17.08±0.34）倍,差异有统计学意义（P＜0.05）.miR-200b mimics上调于预后,QBC939实验组迁移及侵袭能力较阴性对照组明显减弱,下室面细胞数分别减少（8.93±0.76）倍及（13.78±0.34）倍,差异有统计学意义（P＜0.01）；miR-200b mimics上调干预后,QBC939实验组早期凋亡细胞比例较阴性对照组明显增加,为阴性对照组的（4.06±0.54）倍,差异有统计学意义（P＜0.05）.结论胆管癌较癌旁组织miR异常表达,miR-200a/b/c/141在胆管癌组织中显著低表达,且miR-200b上调能明显增加人胆管癌细胞系QBC939的凋亡比例及降低其侵袭能力.

英文摘要：

Objective To detect the role of differentially expressed microRNA （miRNA） in human cholangiocarcinoma and explore their effects on apoptosis and invasiveness of cholangiocarcinoma.Methods The differential expression of miRNA in 3 cholangiocarcinoma patients was detected by miRNA array.The expressions of miR-200a,miR-200b,miR-200c,and miR-141 in human cholangiocarcinoma tissues and normal bile duct tissues were detected by real-time PCR.After transfection with miR-200b mimic,apoptosis and invasiveness of human cholangiocarcinoma cell line QBC939 was evaluated by Annexin-V-FITC dyeing and Transwell assay.Results Comparad with normal bile duct tissues,the number of differential miRNAs in cholangiocarcinoma was 21,including 15 up regulated and 6 down regulated.The expressions of miR200a,miR-200b,miR-200c,and miR-141 in human cholangiocarcinoma tissues were significantly lower than levels in normal bile duct tissues.The invasive ability of QBC939 was decreased after miR-200b mimic transfection.The apoptosis cell number of QBC939 was increased after miR-200b mimic transfection.Conclusion These results indicate that the expression of miRNA is different between cholangiocarcinoma and normal bile duct tissues.Moreover,miR-200a,miR-200b,miR-200c,and miR-141 are likely involved in the invasion and metastasis of cholangiocarcinoma and have potential as a diagnostic and prognostic marker.

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