本试验旨在研究饲料中的玉米蛋白质对军曹鱼幼鱼蛋白质生长的贡献率,并估算军曹鱼幼鱼的碳、氮元素周转时间.试验配制2种等氮等能的饲料,对照饲料以鱼粉为蛋白质源,试验饲料在对照饲料的基础上以玉米蛋白质(玉米蛋白粉形式)替代10%鱼粉蛋白质,分别投喂初始体质量为(21.34±1.54)g的军曹鱼幼鱼24 d后采样,进行碳、氮稳定同位素值测定和数学模型分析.对照组和试验组各设3个重复,每个重复40尾鱼.结果表明:试验组幼鱼的摄食量与对照组无显著差异(P>0.05),而其增重率、饲料转化率、蛋白质效率和特定生长率则均显著小于对照组(P<0.05).试验组幼鱼全鱼碳、氮元素周转半时(以T50表示)(5.72和9.88 d)与对照组(6.03和9.55 d)均无显著差异(P>0.05);而其肌肉碳、氮元素T50(7.79和10.01 d)与对照组(6.99和9.37 d)均有显著差异(P<0.05).玉米蛋白质在军曹鱼幼鱼全鱼和肌肉蛋白质生长中的贡献率分别为7.64%和9.62%,存在显著差异(P<0.05).结果提示,玉米蛋白质对军曹鱼幼鱼蛋白质生长的实际贡献率小于其在饲料蛋白质中所占比例,替代10%鱼粉蛋白质后降低了军曹鱼幼鱼的生长性能和碳、氮元素周转速度,如以其替代军曹鱼幼鱼饲料中的鱼粉(不补充必需氨基酸),替代比例应小于10%.
The purpose of this experiment was to investigate the contribution rate of dietary corn protein to protein growth of juvenile cobia (Rachycentron canadum L.),and estimate the turnover time of carbon and nitrogen elements in juvenile cobia.Two isonitrogenous and isocaloric diets were formulated:the control group diet was prepared with fish meal as a protein source,and experimental diet was prepared with corn protein (corn gluten meal form) to replace 10% fish meal on the basis of the control group diet.After 24 days feeding for juvenile cobia with an average body weight of (21.34 ± 1.54) g,the carbon and nitrogen stable isotope values of sampled fishes were measured and assessed by mathematical model.There were 3 replicates in control group and experimental group,respectively,and each replicate had 40 fish.The results showed that there was no significant difference in feed intake between the experimental group and control group (P 〉 0.05),but the weight gain rate,feed conversion ratio,protein efficiency ratio and special growth rate of fish fed experimental diet were significantly lower than those of fish fed control diet (P 〈 0.05).There was no significant difference between the turnover half life (represented by T50) of carbon and nitrogen for experiment group (5.72 and 9.88 days,respectively) and control group (6.03 and 9.55 days,respectively) in whole fish (P 〉 0.05),and there was a significant difference between Ts0 of carbon and nitrogen for experimental group (7.79 and 10.01 days,respectively) and control group (6.99 and 9.37 days,respectively) in muscle (P 〈 0.05).The contribution rate of corn protein to the protein growth of juvenile cobia whole fish and muscle was 7.64% and 9.62%,respectively,and there was a significant difference between them (P 〈 0.05).It is concluded that the actual contribution rate of dietary corn,protein to the protein growth of juvenile cobia is lower than its percentage in the dietary protein; when 10% fish meal in