研究利用索氏浸提法提取燕麦中皂甙工艺条件。以乙醇-正丁醇为溶剂,以皂甙RC为标准品,采用分光光度法定量分析燕麦提取液中总皂甙含量,采用正交试验对料液比、乙醇浓度、浸提温度对提取率的影响。结果表明,正交试验最佳提取条件为料液比:1∶7;乙醇浓度:70%;浸提温度:70℃,提取率为1.716%;主次顺序依次为:料液比〉浸提温度〉乙醇浓度。该方法操作方便,简单易行,具有推广应用价值。
The technology condition of extracting saponins from oat by the method of soxhlet extraction was studied.The saponins were extracted by ethanol-butanol as the solvent from oat and the content was determined by spectrophotometry.Orthogonal experiment was used to study the extraction technology of saponins in oat.Effects of solid-liquid ratio,ethanol concentration,extraction temperature on extraction rate of saponins were studied.Result,The optimal extracting conditions of oat saponins were established as follows: solid-liquid ratio 1∶7,ethanol concentration 70%,extraction temperature 70℃,ratio 1.716%;The influence order of extraction rate was solid-liquid ratio extraction temperature ethanol concentration.The method has many advantages listed below:convenientin operation,easy access,and possessed of popularization and application value.