目的:考察树脂法对巴戟天低聚果糖中所含色素的脱除能力。方法:以脱色率、寡糖保留率为指标,筛选合适树脂对巴戟天低聚果糖进行脱色,采用单因素试验考察其静态及动态吸附工艺。HPLC—ELSD测定耐斯糖含量。结果:确定采用D941型树脂,其最佳工艺条件为室温(25℃)下,15g·L^-1巴戟天寡糖溶液(pH5.88)4BV,流速4BV·h^-1,径高比1:10。脱色率92.54%,寡糖保留率82.76%,耐斯糖质量分数由22.46%升高至29.94%。结论:D941型树脂对巴戟天低聚果糖具有良好的脱色能力。
Objective: To study on elution property of pigment contained in fructo-oligofructose from Morinda officinalis suitable by resin adsorption. Method: With decolorization rate and retention rate of oligosaccharide as indexes, suitable resin was chosen, and static and dynamic adsorption tests were carried out to investigate decolorization effect of fructo-oligofructose from M. officinalis by single factor test. The content of nystose was determined by HPLC-ELSD. Result: D941 resin was chosen, optimum technology conditions were as follows: room temperature (25 ℃ ), oligosaccharide solution 4 BV (pH 5.88, 15 g· L^-1-l), flow rate 4 BV · h^-1, diameter high ratio 1:10. Under these conditions, decolorization rate and retention rate of oligosaccharide were 92.54% , 82.76% , respectively, and the content of nystose increased from 22.46% to 29.94%. Conclusion: D941 resin had good decolorization effect on fructo-oligofructose from M. officinalis.