本研究以紫花苜蓿为实验对象,以采煤沉陷区退化土壤为培养基质,通过人为伤根模拟矿区沉陷对根系造成的损伤,采用量化模拟方法分析了丛枝菌根真菌在减缓伤根中所起的作用。结果表明,在剪切紫花苜蓿根系后,接菌处理地上干重和根系干重均高于对照处理,在剪切根系程度较小时(1/3和1/2)苜蓿-菌根共生体能够缓解对根系的损伤,但是,剪切根系较大时(2/3和3/4)菌根效应降低,对植物生长发育促进作用减小。接菌改善了紫花苜蓿根际微环境,缓解了根系损伤对植物的负面作用。
In this research we chose alfalfa as an experimental objective and degenerated soil in coal-mining subsidence as culture substrate, to analyze the impact of the fungus of Arbuscular mycorrhizal on reducing injury to the alfalfa roots based on the approach of quantitative simulation that simulates the root damage of the coal-mining subsidence by artificially cutting roots. The results showed that the dry weight above ground and the root dry weight after fungus-inoculated treatment were higher than that of the control was. When a small portion(1/3 or 1/2) of alfalfa root was cut, It was also demonstrated that the alfalfa-mycorrhiza symbiont could reduce injury to plant root in process of the treatment with a small cutting portion(1/3 or 1/2) of alfalfa root; whereas the effect of above mentioned symbiosis on promoting root growth seemed to be diminished in process of the treatment with a large cutting portion(2/3 or 3/4) of alfalfa root. In this research we would conclude that fungusinoculation might improve the status of the rhizosphere microenvironment of alfalfa and relieive the negative effect of the plant growth due to the root damage.