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中文摘要：
目的 探索黄桷树叶总黄酮的提取和初步了解提取物是否具有细胞损伤的保护作用,为黄桷树叶药用价值的研究提供依据。方法 采用乙醇浸提法,提取黄桷树叶中的总黄酮,以芦丁为标准计算提取效率。以鱼藤酮诱导A549A549人肺腺癌细胞损伤为模型,研究提取物对细胞活力、细胞形态、活性氧的产生、凋亡率的影响作用。结果 对黄桷树叶中的总黄酮,60%的乙醇的提取效率为5.02%;提取物32mg/L可明显抑制100μg/L鱼藤酮诱导的细胞活力的降低、细胞形态的改变、活性氧的产生和凋亡。结论 乙醇浸提法能用于提取黄桷树叶中的总黄酮;提取物具有保护鱼藤酮诱导的A549细胞损伤的作用;黄桷树叶具有进一步研究开发其药用价值的潜力。
英文摘要：
Objective To investigate the extraction method of total flavonoids from the leaves oi tlcus lacor ann me protectire effects of extraction on the celtalar damage to provide a basis for the research on the phamaceutical value of ficus [acor leaves. Methods The ethanol extraction method was adopted to extract the total flavonoids in the leaves of ficus lacor and the extraction efficiency was calculated with rutin as the standard. The rotenone induced human lung adenocarcmoma cellular damage served as the model,then the influences of the extraction on the cellular viability, cellular morphology, production of reactive oxygen species （ROS） and apoptosis were researched. Results The extraction efficiency of total flavonoids in the leaves of ficus laeor by 60% eth- anol was 5.02%;the extraction at the concentration of 32 mg/L could significantly inhibit the decrease of cell viability, cellular shape change,ROS prodtletion and apoptosis of A549 cells induced by 100 μg/L rotenone. Conclusion The ethanol extraction method can be used to extract the total flavonoids in the leaves of ficus lacor and the extraction has the protective effects on the A549 cellular dam- age induced by rotenone, the leaves of ficus lacor have the potential for further researching its pharmaceutical value.
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