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中文摘要：
目的探讨阿维A治疗对寻常性银屑病患者血清CC趋化因子配体2（CCL2）、CC趋化因子受体2（CCR2）浓度及皮损CCR2mRNA表达的影响，进一步阐明阿维A对寻常性寻常性性银屑病的作用机制。方法采集30例健康人（正常对照组）及80例阿维A治疗前、后寻常性寻常性银屑病患者（进展期48例，稳定期32例）的外周血和皮肤组织样本，采用酶联免疫吸附法（ELISA）测定血清CCL2和CCR2浓度．通过荧光实时定量PCR检测正常皮肤和皮损CCR2mRNA表达。治疗前及治疗后8周根据皮损面积及严重度指数（PASI）评分观察阿维A疗效，并对治疗前寻常性银屑病患者血清CCL2和CCR2浓度与PASI评分进行相关性分析。结果阿维A治疗寻常性银屑病有效率为78．75％。银屑病组治疗前处于稳定期、进展期的患者血清CCL2，CCR2浓度及皮损CCR2mRNA表达均显著高于正常对照组（P〈0．01），进展期患者血清CCL2，CCR2浓度及皮损CCR2mRNA表达稳定期患者升高（P〈0．01）。银屑病组治疗前血清CCL2，CCR2浓度与PASI评分呈正相关（P〈0．05）。银屑病组治疗后4和8周时血清CCL2，CCR2浓度及皮损CCR2mRNA表达明显低于其治疗前（P〈0．01），且治疗后8周时血时清CCL2，CCR2浓度及皮损CCR2mRNA表达较治疗后4周时降低（P〈0．05）。结论CCL2，CCR2与寻常性性银屑病发生发展密切相关，降低CCL2，CCR2表达可能是阿维A治疗寻常性银屑病的重要机制。
英文摘要：
Objective To explore the effects of acitretin therapy on serum CC chemokine ligand 2 （CCL2） and CC che- mokine receptor 2 （CCR2） levels as well as CCR2 mRNA expression in the skin leisons in patients with pso- riasis vulgaris, thereby further clarifying the action mechanism of acitretin. Methods Peripheral blood and skin tissue samples from 30 healthy people （normal control group） and 80 patients with psoriasis vulgaris before and after acitretin treatment were collected. Among these patients, there were 48 cases with aggressive phase and 32 cases with stationary phase. Serum concentrations of CCL2 and CCR2 were detected using en- zyme linked immunosorbent assay （ELISA）. The expression of CCR2 mRNA in normal and impaired skin tissues was measured using real - time quantitative PCR. The therapeutic efficacy of acitretin was evaluated by Psoriasis Area and Severity Index （PASI） scores before and 8 weeks after acitretin treatment in psoriasis group. Moreover, the correlation of serum CCL2 and CCR2 concentrations with PASI scores were analyzed before acitretin treatment in psoriasis group. Results The effective rate for treatment of psoriasis vulgaris with acitretin was 78.75%. Serum CCL2 and CCR2 concentrations and CCR2 mRNA expression levels in the skin lesions before treatment in psoriasis vulgaris patients with aggressive or stationary phases were higher than those in normal control group （ P 〈 0. 01 ）. These indicators were significantly increased in psoriasis vulgaris patients with aggressive phase when compared with stationary phase （P 〈 0.01 ）. Serum CCL2 and CCR2 concentrations showed positive correlation with PASI scores before treatment in psoriasis group （ P 〈 0. 05）. In comparison with psoriasis vulgaris patients before treatment, serum CCL2 and CCR2 concentrations and CCR2 mRNA expression levels in the skin lesions were significantly decreased at 4 and 8 weeks after treatment （P 〈0. 01 ）. Moreover, these indicators were also lower at 8 weeks than at 4 weeks af
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